背 景

PORC is a protein with protochlorophyllide oxidoreductase activity. This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.

抗原信息

Recombinant protein of Arabidopsis thaliana AT1G03630

同源基因

AT5G54190, AT4G27440, AT1G03630, LOC_Os10g35370, LOC_Os04g58200.

验证应用

Western Blot

预测物种

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

44 / 35 kDa for Arabidopsis thaliana

43 / 37 kDa for Rice

检测图片

上样：约2μg拟南芥和水稻叶片的总蛋白

电泳：12.5% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h