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Anti-VHA-A/V-type proton ATPase catalytic subunit A

Anti-VHA-A/V-type proton ATPase catalytic subunit A

货号: PAB211231

描述: 兔多克隆抗体

基因: AT1G78900

别名: V-ATPase subunit A

验证物种: 拟南芥,水稻

推荐稀释比: WB 1:2500---1:5000

详情描述

 

背 景

The catalytic subunit A of the vacuolar ATP synthase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

抗原信息

Recombinant protein of Arabidopsis thaliana AT1G78900

同源基因

AT1G78900, LOC_Os06g45120, LOC_Os02g07870

验证应用

Western Blot

预测物种

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

69 kDa for Arabidopsis thaliana and Rice

检测图片

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h