背 景

Photosystem I (PSI) is a multisubunit complex that catalyses the final stage of the light reactions in chloroplasts. This protein complex has two main components forming its core complex, PsaA and PsaB. These two subunits bind most of the cofactors required for charge separation and electron transfer in PSI.

抗原信息

Recombinant protein of Arabidopsis thaliana ATCG00350

同源基因

ATCG00350, LOC_Os05g01675, LOC_Os04g16760, LOC_Os01g57964, LOC_Os10g38229, LOC_Os10g21250

验证应用

Western Blot

预测物种

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

83 / 50 kDa for Arabidopsis thaliana and rice

检测图片

上样：约2μg拟南芥和水稻叶片的总蛋白

电泳：12.5% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h