背 景

Malonyl-ACP expressed in developing seeds. Loss of function mutants are embryo lethal and over expression in seeds leads to increased seed oil content.

免疫原

Recombinant protein of Arabidopsis thaliana AT2G30200.

同源基因

LOC_Os03g18590, Os03g0297400， Zm00001d028787, GRMZM2G029543, GLYMA18G06500, etc.

别 名

EMB3147, embryo defective 3147, Malonyl COA-ACP malonyltransferase, MCAMT, MCAT

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol,

推荐稀释比

Western Blot (1:5000)

理论 / 实际 分子量

41.5 / 33 kDa for Arabidopsis thaliana

40.5 / 34 kDa for rice

检测图片

上样： 1ug拟南芥/水稻叶片总蛋白

电泳：12.5% SDSPAGE

转膜： NC (nitrocellulose) 膜， 电压10 v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h