背 景

Shikimate kinase catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.

免疫原

Recombinant protein of Arabidopsis thaliana AT4G39540.

同源基因

AT2G21940， LOC_Os04g54800, Os04g0640600, Zm00001d002128, GRMZM2G004590, GLYMA08G14980, etc.

别 名

Shikimate kinase

验证应用

Western Blot

物种反应性

The sequence of the recombinant protein used for immunization is homologues with the sequence in Arabidopsis thaliana, rice, maize, soybean, rape, pepper, barley, etc.

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol,

推荐稀释比

Western Blot (1:5000)

理论 / 实际分子量

35 / 30 kDa for Arabidopsis thaliana

31 / 29 kDa for  Rice

检测图片

上样： 1ug拟南芥/水稻叶片总蛋白

电泳：12.5% SDSPAGE

转膜： NC (nitrocellulose) 膜， 电压10 v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h