背 景

FtsH5 (VAR1) is a component of the ATP-dependent zinc metallopeptidase. It is involved in the thylakoids biogenesis and in the repair of damaged D1 subunit of photosystem II, a process that protects against cell death under high light conditions. It forms a complex with VAR1. FtsH5 (VAR1) and FtsH1 are interchangeable in thylakoid membranes..

免疫原

Recombinant protein of Arabidopsis thaliana AT5G42270.

同源基因

AT5G42270, AT1G50250, LOC_Os06g51029

别 名

FTSH1, FTSH5, VAR1, Variegated 1

验证应用

Western Blot

物种反应性

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱等

保存条件

理论 / 实际分子量

75 / 75 kDa for Arabidopsis thaliana  and Rice

检测图片

上样：约2 μg 拟南芥/水稻叶片总蛋白

电泳：12% SDS-PAGE

转膜： NC (nitrocellulose) 膜， 电压10 v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h