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Anti-GDH1/ Glutamate dehydrogenase 1

Anti-GDH1/ Glutamate dehydrogenase 1

货号: PAB231209

描述: 兔多克隆抗体

纯度: 血清

基因: AT5G18170

别名: GDH1,GDH 1,GLUTAMATE DEHYDROGENASE 1

推荐稀释比: WB 1:1000---1:5000

详情描述
 
背 景
Encodes the 43 kDa alpha-subunit of the glutamate dehydrogenase with a putative mitochondrial transit polypeptide and NAD(H)- and alpha-ketoglutarate-binding domains. Mitochondrial localization confirmed by subcellular fractionation. Combines in several ratios with GDH2 protein (GDH-beta) to form seven isoenzymes. Catalyzes the cleavage of glycine residues. May be involved in ammonia assimilation under conditions of inorganic nitrogen excess. The enzyme is almost exclusively found in the mitochondria of stem and leaf companion cells.
抗原信息 Recombinant part protein of Arabidopsis thaliana AT5G18170,Q43314
同源基因 AT5G18170
验证应用 Western Blot
预测物种 For more species homologues information, please contact tech support at TEL 400-880-3241
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 44.5 kDa for Arabidopsis thaliana
检测图片  
 
上样:梯度稀释的抗原蛋白
电泳:12% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h