货号: PAB005T
描述: Rabbit polyclonal antibody
推荐稀释比: Western Blot (1:2500-1:5000)
背 景 | |
Anti-Strep-Tag II rabbit antibodyrecognizes Strep-tag II, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal Strep-tag II and is especially suited to Western blot applications. | |
免疫原 | Synthetic peptide corresponding to Strep-tag II(WSHPQFEK). |
验证应用 | Western Blot |
物种反应性 | Species independent |
保存 | 50% glycerol |
检测图片 | |
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上样:大肠杆菌裂解上清 电泳:12.5% SDS-PAGE 转膜: NC (nitrocellulose) 膜,电压10v过夜 封闭:5% 脱脂奶粉,室温孵育1h 一抗: 1:5000 稀释,室温孵育1h |