背 景

GAPDH is a key enzyme in glycolysis (catalyzing D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate). Involved in the maintenance of cellular ATP levels and carbohydrate metabolism.

免疫原

BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g13440

同源基因

 At1g13440, LOC_Os04g40950

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

37 kDa for Arabidopsis thaliana and Rice

检测图片

上样：约2μg拟南芥和水稻叶片的总蛋白

电泳：12.5% SDS-PAGE

转膜： NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h