背 景

GSH2 encodes a protein with similarity to glutathione synthetases, which catalyzes one of the early steps in glutathione biosynthesis. Two transcripts have been detected; the longer transcript is less abundant and the protein is localized to the chloroplast. The smaller transcript, in which the transit peptide is truncated, is localized to the cytosol. Increased glutathione accumulation in response to cesium stress.

抗原信息

Recombinant part protein of Arabidopsis thaliana AT5G27380，P46416

同源基因

AT5G27380

验证应用

Western Blot (WB)，Immunoprecipitation (IP)

预测物种

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

60.3 / 53.6 kDa for Arabidopsis thaliana

检测图片

上样：梯度稀释的抗原蛋白

电泳：12% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h