背 景

HCF244 is a member of the atypical short-chain dehydrogenase/reductase superfamily, a modified group, which has lost enzyme activity. HCF244 interacts with unknown partners in a 200-400 kD membrane associated complex. It activates psbA translation via cis-elements that are confined to the psbA UTRs.

抗原信息

Recombinant part protein of Arabidopsis thaliana AT4G35250，O65502

同源基因

AT4G35250

验证应用

Western Blot (WB)，Immunoprecipitation (IP)

预测物种

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

43.7 / 36.7 kDa for Arabidopsis thaliana

检测图片

上样：梯度稀释的抗原蛋白

电泳：12% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h