背 景

Pyruvate dehydrogenase kinase (PDK) specifically phosphorylates the E1α subunit of the pyruvate dehydrogenase complex (PDC) on a Ser residue using ATP as a phosphate donor. PDK is a unique type of protein kinase having a His-kinase-like sequence but Ser-kinase activity.

抗原信息

Recombinant part protein of Arabidopsis thaliana AT3G06483，Q9SBJ1

同源基因

AT3G06483

验证应用

Western Blot (WB)，Immunoprecipitation (IP)

预测物种

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

41.4 kDa for Arabidopsis thaliana

检测图片

上样：梯度稀释的抗原蛋白

电泳：12% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h