背 景

PREP1 is expressed only in siliques and flowers. It is ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage.

抗原信息

Recombinant part protein of Arabidopsis thaliana AT3G19170，Q9LJL3

同源基因

AT3G19170，AT1G49630

验证应用

Western Blot (WB)，Immunoprecipitation (IP)

预测物种

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

121 / 112 kDa for Arabidopsis thaliana

检测图片

上样：梯度稀释的抗原蛋白

约3 μg拟南芥和水稻叶片的总蛋白

电泳：12% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h