背 景

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH-E1 ALPHA is pyruvate dehydrogenase E1 alpha subunit.

抗原信息

Recombinant part protein of Arabidopsis thaliana AT1G01090，O24457

同源基因

AT1G01090

验证应用

Western Blot (WB)，Immunoprecipitation (IP)

预测物种

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

47.2 / 40.5 kDa for Arabidopsis thaliana

检测图片

Oxidoreductase

上样：梯度稀释的抗原蛋白

电泳：12% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h